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. 2016 Oct 7;7(45):73888–73902. doi: 10.18632/oncotarget.12516

Figure 3. RAP1B is experimentally demonstrated as a direct target of miR-28-5p in renal carcinoma cells.

Figure 3

(A–D) By western blotting analysis, RAP1B protein was significantly downregulated in A498 and ACHN cells transfected with miR-28-5p mimics or plenti-miR-28-5p and was upregulated with miR-28-5p inhibitor treatment. (E–G) By qRT-PCR assay, RAP1B mRNA was not altered after overexpression or knockdown of miR-28-5p in A498 and ACHN cells. (H) Luciferase reporter assays were performed in A498 and ACHN cells by cotransfection of wild type RAP1B-3′-UTR vector or mutant RAP1B-3′-UTR vector and miR-28-5p mimics or negative control. The expression of miR-28-5p mimics significantly suppressed the luciferase activity of the wild type reporter but had minimal effect on the mutant reporter. *P < 0.05; **P < 0.01.