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. 2016 Sep 21;7(45):73960–73970. doi: 10.18632/oncotarget.12170

Figure 5. Stimulation of RHAMM R3-specific T cells by AML patient DCs.

Figure 5

The RHAMM R3-specific cytotoxic CD8+ T cell clone was stimulated for 24 hours without DCs (medium control) or with WT1 EP DCs from HLA-A*0201− (n = 2) or A*0201+ (n = 3) donors. T cells stimulated with T2 cells ± R3 or R5 peptide served as intra-assay controls to validate antigen specificity. IFN-γ was quantified in culture supernatants using ELISA. Data are depicted as mean (+ SD); ns not significant, **P < 0.01, ***P < 0.001, one-way ANOVA with Bonferroni posthoc test.