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. 2016 Sep 22;7(45):74015–74030. doi: 10.18632/oncotarget.12187

Figure 4. BHQ and Orai1 knockdown induce restoration of epithelial phenotype, impair migration and invasion, inhibit cell growth in monolayer cultures and anchorage-independent growth in soft agar of A549 and H1299 cells.

Figure 4

A. Confocal laser scanning microscopy according to the immunolocalization of F-actin, B. CCK-8 cell proliferation assay, C. transwell assays and D. anchorage-independent growth assay of A549 and H1299 cells transfected with Orai1 siRNA for 24 h or stimulated with BHQ (50 μM) for 12 h and then treated with FGF4 (10 ng/mL) for 24 h. A549 and H1299 cells transfected with non-sense control siRNA and without FGF4 or BHQ treatment were used as controls. All graphs represent the mean ± SD of three independent experiments. The axis represents the fold change in the number of cells. ***P < 0.001.