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. 2016 Sep 17;7(45):74247–74258. doi: 10.18632/oncotarget.12093

Figure 1. FMLP-induced chemotaxis signal overrides that of other chemoattractants.

Figure 1

A. The under agarose chemotaxis assay was set up as shown. The neutrophil suspension was placed in the middle well, and FMLP was placed in the left well. C5a, IL-8 or LTB4 was placed in the right well. B-D. Treatments of 0.01, 0.1 or 1 μmol/L fMLP were placed in the left well, and 1 μmol/L C5a, 1 μmol/L IL-8 or 0.1 μmol/L LTB4 were added to the right wells. Representative neutrophil chemotaxis photos are shown, and chemotaxis distances were calculated. The results show that fMLP 0.1 and 1 μmol/L significantly inhibited C5a-, IL-8- and LTB4-induced neutrophil chemotaxis. E. High concentrations of C5a (10 μmol/L), IL-8 (10 μmol/L) and LTB4 (1 μmol/L) exerted no effect on 0.1 μmol/L fMLP-induced neutrophil chemotaxis. The data are expressed as the mean ± SD, n=5 for each group. *P < 0.05 compared with the control group.