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. 2016 Sep 17;7(45):74247–74258. doi: 10.18632/oncotarget.12093

Figure 3. LPS improves fMLP-involved hierarchical neutrophil chemotaxis.

Figure 3

A. The under agarose chemotaxis assay was set as shown. Neutrophil suspension in the presence or absence of LPS was placed in the middle well. Treatment of 0.01 μmol/L fMLP was placed in the left well. Treatments of 1 μmol/L C5a, 1 μmol/L IL-8 or 0.1 μmol/L LTB4 were placed in the right well. B-D. Treatment with 0.01 μmol/L fMLP slightly but not significantly inhibited C5a-, IL-8- or LTB4-induced neutrophil chemotaxis. However, in the presence of 0.001 or 0.01 μg/mL LPS, the inhibitory effect of fMLP was enhanced. Representative neutrophil chemotaxis photos are shown. The data are expressed as the mean ± SD, n=5 for each group. *P < 0.05 compared to the control group.