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. 2016 Sep 30;7(45):74269–74285. doi: 10.18632/oncotarget.12368

Figure 3. Validation of glycolysis proteins in PC-3 and PC-3RR xenografts.

Figure 3

A. Validation of glycolysis proteins using IHC. The glycolysis pathway proteins GLUT-1, PKM1/2 and LDHA, were upregulated in PC-3RR xenografts compared to PC-3 xenografts. Brown indicates positive staining while blue indicates nuclei. Magnification x 400 in all images. B. Validation of glycolysis protein using Western blot. The upregulation of glycolysis pathway proteins GLUT-1, PKM1/2 and LDHA in PC-3RR xenografts were confirmed by Western blot. β-actin was used as a loading control. C. Quantification of Western blot results from PC-3 and PC-3RR xenografts. The results were normalized by the level of β-actin. Results are expressed as mean ± SD (n=3). “*” indicates: p<0.05. Scale bar=50μm.