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. 2016 Sep 30;7(45):74269–74285. doi: 10.18632/oncotarget.12368

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Copyright: © 2016 Hao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. LDHA protein expression in CaP-RR cell lines. Higher levels of LDHA expression were seen in CaP-RR cell lines (PC-3RR, DU145-RR and LNCaP-RR) compared to their matching control cells.β-actin was used as a loading control. B. Quantification of Western blot results from CaP and CaP-RR cell lines. The results were normalized by the level of β-actin. Results are expressed as mean ± SD (n=3) (p<0.05). C. Abundance of LDHA was shown using Progenesis IQ software. D. Validation of LDHA mRNA in PC-3 and PC-3RR xenografts. LDHA mRNA levels were detected using qRT-PCR and was normalized to β-actin level. Significantly increased LDHA mRNA was associated with PC-3RR xenografts (p<0.05). “*” indicates: p<0.05.