Table 1. Regulation of selected genes and extracellular protein levels of human Natural Killer cells in the presence or absence of Aspergillus fumigatus.

human NK cells		gene expression			protein level
	gene (molecule)	x-fold change	2−ΔΔ Ct; NK/NK+Af	P	pg/mL; NK/NK+Af	P
	Cytotoxic molecules
	PRF1 (perforin)	-3	2.1 ± 0.1/0.7 ± 0.1	< 0.0001	16229 ± 1814/17355 ± 1302	ns
	GZMB (granzyme B)	-2.6	0.8 ± 0.1/0.3 ± 0.1	0.001	2752 ± 544/4172 ± 314	ns
	Pro-inflammatory molecules
	IFNG (interferon-gamma)	+38	0.2 ± 0.1/7.6 ± 3.1	0.036	97 ± 49/74 ± 33	ns
	GMCSF (GM-CSF)	+4.6	0.7 ± 0.1/3.2 ± 0.7	0.015	29 ± 14/28 ± 11	ns
	MIP1A (macrophage inflammatory protein 1α)	+3.7	0.6 ± 0.1/2.2 ± 0.5	0.016	1900 ± 768/1891 ± 784	ns
	MIP1B (macrophage inflammatory protein 1β)	+3.7	0.7 ± 0.1/2.6 ± 0.7	0.044	2402 ± 805/1923 ± 448	ns

Relative change of the mRNA expression of the gene of interest after 8 hours of (co-) incubation relative to the housekeeping gene and to time point at hour 0, respectively. The first value represents the results in NK cells incubated alone (NK), the second value the results in NK cells incubated with the fungus (NK+Af) (mean±SEM each). Negative values of “x-fold change” indicate down-regulation, positive values up-regulation.

Protein levels in the supernatant of the molecule of interest were assessed after 8 hours. The first value represents the results in NK cells incubated alone (NK), the second value the results in NK cells incubated with the fungus (NK+Af) (mean±SEM each). Shown are the results of at least five independent experiments.