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. 2016 Oct 18;7(44):71112–71122. doi: 10.18632/oncotarget.12737

Figure 5. Effect of CXCL10 on RM-1 tumor suppression.

Figure 5

A. Conditioned medium of MSCs, MSCs-GFP and MSCs-Sirt1 were applied to detect their chemotactic effect on NK cells via transwell assay. NK cells were counted under a microscope (original magnification: ×200). The data obtained for plotting are from three replicates as mean ± SD. The effect of B. MSCs-GFP and C. MSCs-Sirt1 on NK cells migration was demonstrated through rabbit anti-murine CXCL10 (Abnova). Irrelevant normal rabbit serum at the same dose was applied as control. NK cell count was obtained as described above. D. RM-1 tumor weights were examined in mice injected intraperitoneally with rabbit anti-murine CXCL10 (2.5ug/5ul, Abnova) for CXCL10 depletion. E. Representive tumors were presented. F. Tumor infiltrating cells were isolated from RM-1 tumor tissues and then stained with antibody against NK cells, followed by intracelluar IFN-γ staining. G. Serum IFN-γ level was determined after NK cells depletion. Each group consists of 4 mice. **, P < 0.01; ***, P < 0.001.