Figure 3. Pharmacologic analysis of hGWL-KinDom and hGWL^FL with the AGC kinase inhibitor AT13148.

hGWL-KinDom (•) or immunoprecipitated hGWL^FL (☐) was treated with increasing concentrations of AT13148 and kinase activity was measured by either the Kinase-Glo Max (•) or immunoprecipitation kinase assays (☐). Non-linear regression with Prism 6.0 was used to determine IC₅₀ values.