Figure 2. miRNA subsets associated with the phenotypes of AI resistance and E-inducible apoptosis.

Overall, 205 miRNAs were significantly and relevantly (Holm-adjusted P-value < 0.05 and FC > 1.5 or < 1/1.5) differentially expressed in at least one of the three pairwise comparisons (2A vs WS8, 5C vs WS8, 5C vs 2A). A. Venn diagram of the three comparisons with numbers of significantly and relevantly differentially expressed miRNAs. There are 55 candidates defining the miRNA set relevant for E₂-independent growth (grey, red), 99 miRNAs are 5C-specific (blue, purple), and 30 miRNAs are 2A-specific (yellow, orange). The direct comparison of 5C versus 2A highlights 21 miRNAs (pink). B. Heatmap of the 205 miRNAs comparing WS8, 2A, and 5C with three replicates per cell line. Left color bar matches with colored subsets in A. Within each of the seven color-coded subsets, miRNAs were grouped by complete-linkage hierarchical clustering based on a Pearson correlation distance metric. Corresponding dendrograms are shown.