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. 2016 Sep 18;7(44):71255–71273. doi: 10.18632/oncotarget.12104

Figure 2. GILT inhibits MLV replication.

Figure 2

A. TE671/mCAT1 cells were transfected with pcDNA3.1, wild type GILT, or the DCS mutant, and inoculated with Mo-MLV. Lysates of the inoculated cells were analyzed by western blotting, using the indicated antibodies. B. Amino acid sequences near the thiolreductase active site are indicated. The GILT DCS mutant contains the substitutions of two cysteine residues to serine. C. TE671/mCAT1 cells stably transduced by the empty or shGILT-expressing lentivirus vector were treated with γ-IFN (0.2 μg/ml), and inoculated with Mo-MLV. Three days after the inoculation, Mo-MLV titers were measured by XC plaque assay (n = 3). Error bars show standard deviations. Asterisks indicate statistically significant differences. D. TE671 cells were transfected by pcDNA3.1 or GILT. TE671 cells transduced by the empty or shGILT-expressing vector were treated with γ-IFN (0.2 μg/ml). GILT and actin proteins were detected by western immunoblotting, and the intensities of the proteins were measured by a densitometer. The amounts of GILT were normalized by the actin levels. The amounts of GILT in pcDNA3.1-transfected cells are always set to 1, and relative values are indicated (n = 3).