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. 2016 Sep 23;7(44):71477–71490. doi: 10.18632/oncotarget.12216

Figure 2. Knockdown of NPM1-mA enhances myeloid differentiation of OCI-AML3 cells.

Figure 2

(A) Western blot of NPM1-mA and NPM1 in the nuclear and cytosolic fractions of OCI-AML3 infected with empty vector or the shNPM1 lentivirus, HL60 cells served as control. β-actin (cytosolic) and H2A.X (nuclear) served as the loading controls. (B) Flow cytometry showing the percentage of the CD14-positive cells in infected OCI-AML3 cells following PMA treatment for 72 h. (C) Representative Wright-Giemsa staining image of infected OCI-AML3 cells followed by PMA treatment. (D) Percentage of differentiated cells determined by counting at least 200 cells on the slides under a light microscope. Three independent experiments were performed. *P < 0.05.