Figure 1. In vivo biopanning of a Llama phage library in an animal model of orthotopic glioma.

Anti-M13-p8 (A) and anti-CD34 (B) immunostainings of sections of E98 xenografts in mouse brain after intravenous injection of 10¹² phages of the nanobody-phage display library, and cardiac perfusion. Note that phages are associated with tumor vasculature, but to a lesser extent with blood vessels in normal brain. N = normal, T = tumor. Bars correspond to 100 μm.