Figure 2. C-C7 recognizes tumor vessels in glioma xenografts and brain metastasis.

Immunohistochemical analysis of nanobody C-C7 in a diffuse-invasive part of cerebral E98 xenografts (A), normal mouse brain parenchyma (B), a representative brain metastasis of Mel57-VEGF₁₆₅ melanoma (C) and a Mel57-VEGF-A165 xenograft after treatment with the VEGFR2 inhibitor vandetanib [42] (D). C-C7 recognizes subsets of tumor vessels in cerebral E98 lesions, while normal mouse brain vessels are negative. Insets in A and B show CD34 immunostainings of serial sections. Note that inhibition of VEGFR2 activity in panel D results in loss of C-C7 reactivity. N = normal, T = tumor. Bars correspond to 50 μm.