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. 2016 Sep 28;7(44):71754–71760. doi: 10.18632/oncotarget.12330

Figure 2. Effects of myricetin on the ratio of TIMP1/MMPs in diabetic fibroblasts Legends.

Figure 2

(Panel A). The normalized mRNA expression level of MMPs in diabetic fibroblasts in the presence of 3 μM myricetin. (Panel B). The normalized mRNA expression level of TIMPs in diabetic fibroblasts in the presence of 3 μM myricetin. (Panel C). The TIMP/MMP ratio in diabetic fibroblasts in the presence of 3 μM myricetin. These results show a decrease in the mRNA expression levels of MMP1, MMP2 and MMP9 in diabetic fibroblasts treated with 3 μM myricetin. After treatment, the mRNA expression level of MMP1 decreased by 67% (P < 0.05), the expression level of MMP2 decreased by 51% (P < 0.05), and the expression level of MMP9 decreased by 68% (P < 0.05). The mRNA expression level of TIMP1 was analyzed by qRT-PCR, and the data show that cells treated with myricetin had an mRNA expression increase of 72% (P < 0.05) compared with cells without myricetin incubation. Condition: The mRNA analysis kits for MMP1, MMP2 and MMP9 were supplied by the Shanghai Sangon Institute of Biology, and the mRNA analysis kit for TIMP1 was supplied by R&D Systems. The 2−ΔΔCT method was used to analyze relative changes in gene expression.