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. 2016 Sep 30;7(44):71790–71801. doi: 10.18632/oncotarget.12374

Figure 3. JWA decreases the sensitivity of GC cells to lapatinib.

Figure 3

(A) Expressions of HER2 and JWA were examined in whole-cell lysates by Western blotting. (B and C) SGC-7901 cells with or without JWA knockdown (B) and NCI-N87 cells with or without JWA overexpression (C) were treated with the indicated doses of lapatinib for 24 h. Cell survival was determined using the CCK8 assay. The cell survival rates are presented as means ± SD from three independent experiments. (D) SGC-7901 cells were transfected with si-JWA or its vector for 48 h, followed by incubation with 30 μM lapatinib for 24 h, and then analyzed by flow cytometry. (E) NCI-N87 cells were transfected with Flag-JWA or its vector for 48 h, followed by incubation with 1 μM lapatinib for 24 h, and then analyzed by flow cytometry. (F and G) Quantification of apoptosis in D and E. Columns indicate average of triplicates and bars indicate SD. *P < 0.05, **P < 0.01.