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(A) Suppressive effect of UA in 8 different signaling pathways. (B) Immunohistochemical staining of mice tumor tissues. UA reduced expression of HIF-1α, VEGF, PCNA and β-catenin in tumors. (×400 magnification). (C) Immunofluorescence staining of MDA-MB-231 cells. HIF-1α distribution in MDA-MB-231 cells after 24 h treatment with UA (×200 magnification). DAPI (blue) and anti-mouse FITC to detect HIF-1α (green).
