Figure 5. Effect of UA on HIF-1α and VEGF expression in breast cancer cells.

(A) The mRNA expression levels of HIF-1α, VEGF and related factors in MDA-MB-231 cells after HIF-1α over expressed. (B) MDA-MB-231 cells were treated with CoCl₂ (a chemical inducer of HIF1). (C) The protein expression levels of HIF-1α and VEGF on HIF-1α or VEGF over expressed MDA-MB-231 cells were examined by western blotting. (D) The related protein levels in HIF-1α over expressed 4T1 cells.