Figure 2. Synergistic effect of combination treatment.

(A) Combination of cisplatin and scriptaid at sub-lethal doses showing significantly enhanced cytotoxicity than either of single agent treatment and high dose cisplatin. The combination remained effective in both condition of hypoxia- 1% and 0.2%. (B) MTT assay of H460 cells shows that the combination treatment was significantly more effective than either of the single agent in both normoxic and hypoxic conditions. (C) Cleavage of PARP as seen by western blot analysis as a marker for cell death. High dose cisplatin and combination treatments resulted in greater amount of cell death in all conditions. Graphs represent the fold change in expression of cleaved PARP, normalized with the loading control- β-actin. C= untreated control, L=2μg/ml cisplatin, M= 5μg/ml cisplatin H= 7.5μg/ml cisplatin, S1= 1μg/ml scriptaid, S2= 2μg/ml scriptaid. * indicates p < 0.05, ** indicates p < 0.005.