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. 2016 Oct 4;7(44):71987–71997. doi: 10.18632/oncotarget.12453

Figure 3. Mad2-mediated SAC inactivation in Spc24-depleted oocytes.

Figure 3

Control and Spc24-depleted oocytes were fixed at 5 h following release from IBMX. (A) Oocytes were immunostained with anti-Mad2 antibody (green) and Hoechst 33342 (red). Scale bars: 20 μm. (B) Oocytes were immunostained with anti-Bub3 antibody (green) and Hoechst 33342 (red). Scale bars: 20 μm. Quantification of fluorescent intensity of Mad2 or Bub3 is shown in the right panel of images, respectively. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05). The total numbers of analyzed oocytes are indicated (n).