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. 2016 Oct 4;7(44):71987–71997. doi: 10.18632/oncotarget.12453

Figure 4. Loss of Spc24 causes misaligned chromosomes in meiotic oocytes.

Figure 4

(A) Abnormal chromosome alignment in MII oocytes after microinjection of Spc24 siRNA. In the control group, most oocytes showed normal chromosome alignment, while in the Spc24-depleted oocytes, most oocytes showed severely misaligned chromosomes. α-tubulin (green); DNA (red). Scale bars: 20 μm. (B) The rates of oocytes with misaligned chromosomes in the siRNA injection and control group. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05). (C) Oocytes in MI were stained with anti-tubulin, ACA and Hoechst 33342. Scale bars: 20 μm. (D) Metaphase plate width was determined by measuring the axis distance between the two lines at the edges of the DNA. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05). The total numbers of analyzed oocytes are indicated (n).