Figure 6. Correct kinetochore-microtubule attachment depends on Spc24.
(A) Control and Spc24-depleted MI oocytes were cold-treated and stained with anti-tubulin (microtubules, green), ACA (kinetochores, purple), and Hoechst 33342 (chromosomes, blue). Enlarged views for the kinetochore-microtubule attachments in the right panel. Aa: normal attachment; Ab-e: loss of attachment. Scale bars: 10 μm. (B) Quantitative analysis of kinetochore-microtubule attachments in control and Spc24-depleted oocytes. Data are expressed as mean ± SEM of at least 3 independent experiments. *Significantly different (P < 0.05). The total numbers of analyzed oocytes are indicated (n).