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. 2016 Sep 27;7(44):72084–72098. doi: 10.18632/oncotarget.12290

Figure 4. Effects of MTDH silencing in lung SCC cell lines.

Figure 4

A. MTDH mRNA expression was evaluated by qRT-PCR in SK-MES-1 and EBC-1 72 h after transfection with si-MTDH-1 or si-MTDH-2. GUSB was used as an internal control. B. MTDH protein expression was evaluated by Western blot analysis in SK-MES-1 and EBC-1 72 h after transfection with miR-145-5p or miR-145-3p. GAPDH was used as a loading control. C. Cell proliferation was determined with the XTT assays 72 h after transfection with 10 nM si-MTDH-1 or si-MTDH-2. *P < 0.05. D. Cell migration activity was determined by wound healing assays. *P < 0.001. E. Cell invasion activity was determined using Matrigel invasion assays. *P < 0.001.