Fig 2. Ste12 may act upstream of TORC1. Altered vacuole size defects do not account for the nitrogen stress-response defect of ste12^PIKFYVE.W1037^STOP mutant.

(A,B) Nitrogen stress (upper panel) and rapamycin (bottom panel) response of ste12^PIKFYVE-W1037^STOP and wild-type cells. The frequencies of mitotic and dividing cells along with changes in cell length at division are shown (C) Cells grown exponentially at 28°C in EMMG were stained with FM4-64. ste12^PIKFYVE.W1037^STOP and an alternative vacuole mutant pxa1.Δ exhibit enlarged vacuoles compared to wild-type. (D) pxa1.Δ can respond to nitrogen stress as efficiently as wild type.