Fig 3. Autophagy defects is unlikely to account for the nitrogen stress-response defect of ste12^PIKFYVE.W1037^STOP mutant.

(A) ste12^PIKFYVE.W1037^STOP cells are autophagy-deficient. Cells were starved of nitrogen for the indicated times and then plated on YES media as 10-fold serial dilutions. (B) Autophagy-deficient atg1.Δ can respond to nitrogen stress as efficiently as wild type. (C) Ste12-defficient strains are sensitive to Torin1 and Brefeldin A. Exponentially growing cultures in EMMG were plated on indicated media as 10-fold serial dilutions.