Fig 5. TORC1 remains on vacuoles during nitrogen stress in both wild type and ste12^PIKFYVE.W1037^STOP.

(A) Western blot of protein extracts from exponentially grown cells. TORC1 activity of ste12^PIKFYVE.W1037^STOP is inhibited by nitrogen stress similarly as in control cells as determined by immunoblotting for Maf1 phosphorylation. (B) Live cell imaging at indicated time-points of exponentially growing cultures shifted from media containing glutamate to proline as a nitrogen source. Mip1^RAPTOR.GFP remains localized to vacuoles in the ste12^PIKFYVE.W1037^STOP mutant.