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. 2017 Mar 8;12(3):e0173465. doi: 10.1371/journal.pone.0173465

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© 2017 Mohanty et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (2A) Agarose gel electrophoresis of PCR products from amplification experiments with Juniperus pollen DNA tested with species-specific J. ashei primers (asheimatKF1 and asheimatKR1). Lane 1: Juniperus ashei pollen DNA, Lane 2: Juniperus pinchotii pollen DNA, Lane 3: Juniperus virginiana pollen DNA, Lane 4: Blank, Lane 5: Negative control, Lane 6: λ-DNA ladder (HindIII) as marker (M). (2B) Agarose gel electrophoresis of PCR products obtained from the DNA extracted from the Buck bio-slide sampler from Canada on 15 January 2014 and tested with the primers asheimatKF1 and asheimatKR1. Lane 1: Blank (No DNA), Lane 2: Juniperus ashei pollen DNA obtained from Canada slide on 15 January 2014, Lane 3: Blank (No DNA), Lane 4: Positive control, Lane 5: Negative control (only milliQ water and no DNA), Lane 6–7: Blank (No DNA), Lane 8: exACTGene^™ mid- range (300-5000bp) DNA Ladder.