Fig 3. Protein expression levels in homogenized cardiac tissue from RV and LV of rats subjected to acute exhaustive treadmill exercise compared to sedentary controls, n = 9.

(a, b) Protein expression levels in (a) RV and (b) LV from sedentary controls (white bars) and exhaustively exercised rats (black bars); (c, d) Exemplary western blots of protein expression levels of SERCA2a (panel 1), β-tubulin (panel 2), PLB (panel 3) and (c) left pPLB Ser16 (panel 4) and combined PLB (green) and pPLB Ser16 (red) (panel 5), right pPLB Thr17 (panel 4) and combined PLB (green) and pPLB Thr17 (red) (panel 5) in RV, (d) left pPLB Ser16 (panel 4) and combined PLB (green) and pPLB Ser16 (red) (panel 5), and right pPLB Thr17 (panel 4) and combined PLB (green) and pPLB Thr17 (red) (panel 5) in LV. SERCA2a, sarcoplasmic reticulum Ca²⁺-ATPase 2a; PLB, phospholamban; pPLB Ser16, phospholamban phosphorylated at serine 16; pPLB Thr17, phospholamban phosphorylated at threonine 17; Ctrl, control; Ex, exhaustively exercised. * p < 0.05.