Fig 1. Morphological aspects of morphogenesis and plant regeneration in Triticum monococcum L.

(A-C). Effect of PGRs on the regenerable callus induction from immature embryos on the medium supplemented with 3 mg/L DIC (A), 3 mg/L DIC and 25 mg/L DAM (B), 3 mg/L DIC, 50 mg/L DAM and 0.25 mg/L TDZ (C), 6 weeks of culture. (D-H, J-K) Details of morphogenesis in immature embryo cultures induced on the medium supplemented with 3 mg/L DIC, 50 mg/L DAM and 0.25 mg/L TDZ; (D) swelling at the inner part of cultured immature scutellum, 7 days of culture; (E) appearance of nodular structures on the scutellum, 14 days of culture; (F) formation of leaf-like structures (asterisks), 20 days of culture, and (G) their development by the end of the callus induction phase; (H) differentiation of multiple plantlets on regeneration medium under 16 h of photoperiod, 40 days of culture; (J) shoot apices formation at the base of leaf-like structure on regeneration medium, 50 days of culture; (K) pro-morphogenic granular masses (black arrows) appeared on the same explant concomitantly with the development of plantlets and leaf-like structures (asterisks), 35 days of culture. (I) Callus induced on the medium supplemented with 3 mg/L DIC and 50 mg/L DAM displaying the formation of non translucent scutellum-like (white arrows) and translucent off-white nodular (black arrows) morphotypes, 30 days of culture. (L) In vitro plant regeneration from morphogenic callus, 25 days of culture on medium lack of growth regulators under 16-hour photoperiod. (M) Mature regenerated plants growing under greenhouse conditions.