Fig 2. Relative resistance of diverse HCVpp to neutralization by HC33.4 and AR4A shows a significant positive correlation, which is not the result of shared binding sites.
(A) Each point indicates mean fraction unaffected (Fu) of a single HCvpp by 10 μg/mL of HC33.4 on the x-axis and AR4A on the y-axis, measured in duplicate. Fu is infection in the presence of 10 μg/mL of bNAb/infection in the presence of nonspecific human IgG. R- and p-values determined by Spearman correlation. (B) HC33.4 and AR4A do not compete for binding to E1E2. Values represent binding of biotinylated bNAbs to 1a154 (H77) E1E2 in an ELISA in the presence of the indicated blocking antibody relative to binding in the absence of blocking antibody. Combinations showing >50% reduction in binding of the biotinylated bNAb are marked in red.