Fig 3. Cytokine profile developed after immunizations in vaccinated mice.
Spleen cells were taken from each mouse 20 days after the last immunization. After stimulation with Tc52, IFN-γ, IL-4 and IL-17 producing cells were measured by ELISPOT. Results are expressed as spot forming units (SFU) normalized to 106 spleen cells, showing the mean and standard deviation of each group. In the table, the mean of normalized SFU of each cytokine is expressed as percentage of the sum of the three cytokines measures in the group to estimate the relative secretion of each cytokine, no the cells producing them. The change in cytokine profile in immunizations with c-di-AMP, with or without αCGP, is highlighted in bold. Results are representative of two independent experiments. **P < 0.01; ***P < 0.001.