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. 2017 Feb 24;11(2):e0005300. doi: 10.1371/journal.pntd.0005300

Fig 4. Parasitemia after lethal T. cruzi challenge.

Fig 4

Twenty days after the last immunization, mice were lethally challenged with the 1000 RA strain bloodstream trypomastigotes. Levels of parasites in blood were measured in each mouse every 2 or 3 days. A. Mice were immunized by intranasal route with Tc52+c-di-AMP, NTc52+c-di-AMP, or CTc52+c-di-AMP, and the control group received c-di-AMP alone. B. Groups immunized with NTc52 and different adjuvants: NTc52+c-di-AMP, NTc52+c-di-AMP+αGCP, NTc52+αGCP, a prime-boost strategy: NTc52PB (previously described in Matos 2016), and both control groups (c-di-AMP and αGCP). C. Area under the curve (AUC) of parasitemia of all immunized groups related to AUC of control group (times lower than control). c-di-AMP was taken as control group, except for group NTc52+αGCP. For group NTc52+c-di-AMP+αGCP, the comparison was done with both controls (c-di-AMP and αGCP), with similar results. D. Statistical analysis at each point of parasitemia curves. Statistical significance between control group (c-di-AMP) and other groups is indicated. Results are representative of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns: non-significant.