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. 2017 Feb 24;13(2):e1006232. doi: 10.1371/journal.ppat.1006232

Fig 3. Hepatitis C virus genomic RNA selectively inhibits PTPRE expression.

Fig 3

Huh 7.5 cells were mock transfected or transfected with full-length, in vitro transcribed HCV genomic RNA (gRNA) (A). HCV E2, PTPRE, VAPA and Grb2 protein levels were analyzed 96 hours later by immunoblot analysis (A). GAPDH served as the loading control. PTPRE (B), VAPA (C), and Grb2 (D) have 7 to 8 nt sequences complementary to the highly conserved HCV 8 nt sequence. There is 38% complementarity between the HCV RNA sequence examined and VAPA, Grb2, and Site 1 of PTPRE, and 56% complementarity with Site 2 of PTPRE. PTPRE, VAPA and GAPDH expression were measured 96 hours post transfection by immunoblot analysis. Experiments were performed three times with consistent results.