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. 2017 Mar 8;12(3):e0173549. doi: 10.1371/journal.pone.0173549

Fig 4. Proliferative response of PBMC lymphocytes to crustacean extracts.

Fig 4

Roliferation of total (A), CD4+ (B), CD8+ (C) and CD56+ (D) lymphocytes in response to raw blue swimmer crab (RC1), cooked blue swimmer crab (CC1), raw black tiger prawn (RP1), cooked black tiger prawn (CP1), raw mud crab (RC2), cooked mud crab (CC2), raw banana prawn (RP2), cooked banana prawn (CP2) extracts is shown as assessed by CFSE staining at day 7 of culture. ‘No antigen’ was used as a negative control and tetanus toxoid (TT) as a positive indicator of proliferation. Box and whisker plots of crustacean-allergic (blue; n = 8) and non-atopic (red; n = 4) subject data represent minimum to maximum (whiskers), interquartile range (box) and median (bar) values (based on maximal proliferation for either 50 or 100 μg/mL extract). * p <0.05, ** p <0.01, *** p <0.001 (Friedman’s test with Dunn’s post hoc correction, combined allergic and non-atopic data).