Fig 5. Representative flow cytometry gating strategy to assess immune cell response to crustacean extracts.

A representative flow cytometry gating strategy for analysing proliferation and cytokine profiles of lymphocyte subsets in crustacean extract-stimulated PBMC cultures is shown. Cells were first gated on live, total lymphocytes (A), then CD4⁺, CD8⁺ or CD56⁺ lymphocytes (B). Next, proliferated (CFSElo) cells for each subset (e.g. CD8⁺) were identified and analysed for intracellular IL-4 and IFN-γ expression (C).