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. 2017 Jan 17;139(6):2338–2344. doi: 10.1021/jacs.6b11366

Table 1. Absorbance Maximum (λmax) Values and Proton Pumping Scores of the Analogue Pigments Generated in This Study.

      H+ c
opsin retinal analogue λmax (nm)a white 617 nm 660 nm 730 nm
  A1 520 +++ + – –
PR A2 552 +++ +++ + –
  MOA2 585 ++ – nd nd
  DMAR 558 – + nd nd
  MMAR 567 – + + ++
             
  A1 540 ++ nd – –
PR-DNFS A2 566 + nd – +
  MMAR ∼740 – nd – ++
             
  A1 536 + nd + –
PR-TA A2 563 + nd + +
  MMAR 561b – nd + ++
             
  A1 540 ++++ +++ ++ –
  A2 566 ++++ ++++ ++++ +
GR MOA2 620 ++ – nd nd
  DMAR 538b + + nd nd
  MMAR 570 + + ++ ++
             
  A1 549 ++++ ++++ +++ –
  A2 571 +++ nd ++++ ++
GR-FS MOA2 630 ++ – – –
  MMAR 571b + + ++ ++++
a

λmax of purified protein.

b

λmax from hydroxylamine difference spectrum in vesicles.

c

Proton pumping activity normalized within each light condition to the highest pumping rate measured under that condition: ++++, 100–70%; +++, 70–40%; ++, 40–20%; +, 20–5%; −, <5% (see Selected Materials and Methods for relative rates between sets and further explanation). nd = not determined.