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. 2016 Aug 5;7(34):54303–54316. doi: 10.18632/oncotarget.11103

Figure 4. Effect of binge ethanol exposure on cell proliferation in the pancreas.

Figure 4

A. The proliferating cells were determined with Ki67 IHC. The representative images revealing nuclear staining of Ki67 are shown. Bar = 100 μm in the top panel (magnification of 20X) and Bar = 100 μm 50 μm in the bottom panel (magnification of 40X). B. Twenty fields covering at least 1,000 cells were randomly selected and Ki67 positive cells were counted at 40X magnification. Three-four animals were analyzed for each group. The percentage of Ki67 positive cells was calculated and expressed relative to the control. Each data point was the mean ± SEM of three independent experiments. ** denotes significant difference (p < 0.01) from the control.