Figure 1. CoCl₂ treatment increases expression of HIF-1/2α and PHD2.

A. HK-2 cells were incubated with 200 μM of CoCl₂ for applied time, mRNA and protein expressions of HIF-1α, HIF-2α and PHD2 were tested by RT-qPCR assay (A) and Western blot assay B., respectively. HIF-1α, HIF-2α and PHD2 mRNA expression was normalized to 18S (n = 12). HIF-1α, HIF-2α and PHD2 protein expression was normalized to β-actin (B, lower panels, n = 14). *P < 0.05 vs. 0h group; # P < 0.01 vs. 0h group.