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. 2016 Jun 9;7(34):54430–54444. doi: 10.18632/oncotarget.9914

Figure 3. In cell association of Ku with SAF-A is regulated by SAF-A phosphorylation at Ser59.

Figure 3

A. Presence of Ku and DNA-PK increased post irradiation (3Gy, 1 h) in the SAF-A IP isolated from HEK293 cells. B. Dynamics of Ku association with SAF-A after irradiation; Ku level was measured in the FLAG-SAF-A IP from HEK293 cells. C. Increased association of SAF-A and pS59-SAF-A in the FLAG-Ku IP after irradiation of HEK293 (upper panel), and U2OS cells (bottom panel). D. Kinetics of association of SAF-A and pS59-SAF-A with FLAG-Ku in HEK293 cells. E. Ku binds to WT SAF-A and the phosphomimetic S59D mutant in chromatin but not to the non-phosphorylatable S59A mutant.