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. 2016 Jul 17;7(34):54445–54462. doi: 10.18632/oncotarget.10642

Figure 10. Abrogation of the type I IGF receptor prevents progression through the cell cycle and inhibits Ras/Raf/MAP-kinase pathway activity.

Figure 10

SNU-1, MKN74, NUGC3 and AGS cells were transfected, plated onto coverslips and cultured in serum-containing medium for three days. Cells were assayed for BrdU incorportation A. or histone H3 phosphorylation B. SNU-1, were processed in suspension. Representative photomicrographs and the proportion of cells in the S-phase or the mitotic-phase of the cell cycle are shown; asterisks indicate that the proportion of cells in either phase of the cell cycle is significantly lower after transfection with si IGF-IR 2 than with the scrambled oligonucleotide (Unpaired t-test; S-phase: SNU-1, p = 0.0078; MKN74, p = 0.0001; NUGC3, p = 0.0001; AGS, p = 0.0004 and mitotic-phase; SNU-1, p = 0.0009; MKN74, p = 0.0046; NUGC3, p = 0.0023; AGS, p = 0.017). SNU-1, MKN74 and NUGC3 cells were transfected with scrambled oligonucleotide (scr.) or si IGF-IR (siR), cultured in serum-containing medium, lysed and type I IGF receptor, phosphorylated ERK1 and ERK2, total ERK1 and ERK2 and GAPDH were measured (C).