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. 2016 Jul 9;7(34):54583–54595. doi: 10.18632/oncotarget.10518

Figure 3. Treatment with NVP-BGJ398 does not induce NRH-LS1 cells to undergo apoptosis or senescence in a manner to account for the reduced cell number.

Figure 3

(A) The number of cells with active caspase 3/7 during 96 h of treatment with 100 nM of NVP-BGJ398. (B) The percentage of apoptotic cells after treatment with NVP-BGJ398; shown one representative experiment (n = 3), error bars represent the standard error (SE) of the final measurement. (C) Increase in SA-β-galactosidase activity between cells treated with 100 nM of NVP-BGJ398 (red) and untreated (DMSO) cells (blue). Representative flow cytometry histograms of n = 3 biological replicates shown. (D) Representative image of SA-β-galactosidase staining after 72 h of treatment with 100 nM NVP-BGJ398. Senescent cells marked with arrows. Scale bars represent 50 μm. (E) The percentage of senescent cells after treatment with NVP-BGJ398 based on flow cytometry assay (n = 3), error bars represent the standard deviation (SD) from three independent experiments.