Figure 6. TGFA promotion of osteosarcoma cell growth in vitro.
(A) TGFA knockdown was achieved by si-TGFA as demonstrated by Western blot assay which showed much less protein expression of TGFA. (B and C) MTT assay results showed that OS cell growth was attenuated in response to TGFA inhibition by si-TGFA. (D and E) BrdU results showed that DNA synthesis capacities were reduced after TGFA inhibition. Data are presented as mean ± SD of three independent experiments.