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. 2016 Jul 19;7(34):54897–54912. doi: 10.18632/oncotarget.10703

Figure 5. SGI-1776 displays anti-tumor activity in combination with a BRAF inhibitor in 3D melanoma models.

Figure 5

(A) Western blot analysis showing endogenous levels of PIM-1, −2, and −3 in human melanoma cell lines, normal fibroblasts, and cell lines rendered resistant to BRAF inhibitors (BR). Hsp90 served as loading control. (B) Collagen-embedded melanoma spheroids were treated for 72 h with SGI-1776 [10 μM] or PLX4720 [5 μM] as single agents or in combination. Spheroids were then stained for live (green) and dead (red) cells. Experiments were conducted in triplicate and representative images are shown. Scale bar represents 150 microns. (C) PIM1 gene expression in melanoma patient samples pre-treatment and upon treatment progression on dabrafenib or vemurafenib. Total RNA was isolated from fresh frozen melanoma tumors in 21 patients (GSE50509). The graph shows data for 12 patients with greater PIM1 gene expression in progressing tumor samples (Prog) than in pretreated samples (Pre). The red and blue bars represent the normalized microarray expression of PIM1 (on a log scale) in Pre and Prog samples, respectively.