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. 2016 Jul 19;7(34):54998–55011. doi: 10.18632/oncotarget.10714

Figure 1. miR-328 is downregulated in PDGFBB-stimulated PASMCs.

Figure 1

Human pulmonary artery smooth muscle cells (PASMCs) were starved for 12 h in starvation condition (0.2% FBS) before treating with PDGFBB (20 ng/mL) for 24 h. The differential expression of 1,087 miRNAs were profiled using a sensitive S-Poly(T) Plus qRT-PCR method (A), leading to the identification of 9 downregulated (B) and 6 upregulated miRNAs (C). The downregulation of miR-328 was further confirmed in rat PASMCs (D), displaying a time- and dose-dependent manner upon PDGFBB treatment (E and F). MiR-328 expression was specifically inhibited by PDGFBB rather than other growth factors including AngII, TGF-β, IGF, VEGF, ET-1, PDGFAA and FGF at concentrations indicated (G). Data are expressed as means ± SD with at least three independent experiments. *p < 0.05, **p < 0.01 compared to control without PDGFBB treatment (PDGFBB-).