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. 2016 Jul 19;7(34):54998–55011. doi: 10.18632/oncotarget.10714

Figure 6. The inhibition of miR-328 by PDGFBB is associated with increased DNMT1 expression.

Figure 6

PASMCs were pretreated with 5-aza-2′-deoxycytidine (5-aza-dC) in a concentration of 5 mM for 48 h and then stimulated with PDGFBB. MiR-328 expression was determined by qRT–PCR (A). The expression of DNMT1, DNMT3A and DNMT3B at mRNA and protein levels were measured in PDGFBB-treated PASMCs using qPCR and western blotting (B and C). DNMT1 protein level in PASMCs infected with shDNMT1 lentivirus (D). After the infection of shDNMT1 levtivirus, miR-328 expression was measured in PASMCs in the absence and presence of PDGFBB (E). Results are shown as means ± SD of three independent experiments. *p < 0.05 compared to control without PDGFBB treatment (PDGFBB-), and #p < 0.05 compared to control lentivirus with PDGFBB treatment.