Figure 1. Anti-leukemic effect of MLN0128 in AML.

AML cell lines A, B. and AML progenitor cells C. were treated with different concentrations of MLN0128 for 72 hours. Growth inhibition of cell lines was measured by Vi-Cell XR cell viability analyzer. Apoptosis induction of cell lines and primary progenitor cells were measured by flow cytometry. Specific apoptosis was calculated as described in the Materials and Methods. Clinical information on primary AML samples is included in the Supplementary Table S1 apoptosis section. *: Cell growth was significantly inhibited at the lowest concentration of MLN0128 on all cell lines examined (Student t-test, p < 0.05).