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. 2016 Jul 13;7(34):55395–55408. doi: 10.18632/oncotarget.10577

Figure 4. MiR-150-5p overexpression decreases hypersensitivity of JMML bone marrow (BM) mononuclear cells to GM-CSF.

Figure 4

A. MiR-150-5p expression levels in BM mononuclear cells from two JMML patients infected with miR-150-5p overexpressing lentivirus or empty vector (Control) evaluated by qRT-PCR. Expression levels of U44 were used to normalize the raw miR-150-5p values. B. In vitro proliferation assay of BM mononuclear cells from three newly diagnosed JMML patients after infection with miR-150-5p lentivirus or empty vector (control). After infection with GFP expressing constructs, GFP-sorted cells were mixed with non-GFP cells at a 4 to 1 ratio. GFP-positivity was evaluated by flow cytometry. The frequency of GFP positive cells in control versus miR-150-5p overexpressing JMML cell populations was calculated for a maximum period of 7 days after sorting the cells.