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. 2016 Aug 1;7(34):55473–55490. doi: 10.18632/oncotarget.10980

Figure 1. Co-culture of macrophages with nasopharyngeal carcinoma cells increases proinflammatory cytokine expression.

Figure 1

Relative gene expression levels of proinflammatory cytokines, Il6, Tnfa, Il-1b; chemokine Mcp-1; anti-inflammatory cytokine Il-10 and protein levels of IL-1b were measured from undifferentiated THP1 cells co-cultured with C666-1 at both (A) early and (B) late/extended time points. Relative gene expression levels were determined by quantitative real-time PCR (qPCR). Protein expression of IL-1b was determined by ELISA. The images shown are a representative set from 2-3 experiments *P < 0.05, **P < 0.01, ***P < 0.001 (mean ± S.D, n = 3).