Figure 3. Comparison of gene expression between contact and non-contact co-cultures of THP-1 and NPC cells.

For contact co-culture, THP-1 cell were added directly into culture wells containing C666-1 cells. For non-contact (transwell) co-culture, 0.2 × 10⁶ C666-1 cells were cultured in the lower well of a 6-well plate and 0.2 × 10⁶ THP-1 cells were seeded in the upper inserts. The co-cultures were maintained for 3 hrs or 48 hrs. Relative expression of genes related to (A) inflammation, and (B) cancer-initiation and metastasis, was determined by qPCR. The data shown is representative of set from 2 experiments *P < 0.05, **P < 0.01, ***P < 0.001 (mean ± S.D, n = 3).